ybfQ [2019-08-27 12:35:47]

unknown

locus: BSU_02330

pI: 4.91

mw: 37.11 kDa

protein length: 322 aa

gene length: 969 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

Gene

Coordinates: 252,514 → 253,482

The protein

Protein family

• UPF0176 family (single member, according to UniProt)

[SW|Domains]

• Rhodanese domain (aa 125-219) (according to UniProt)

Structure

• [PDB|4F67] (from Legionella pneumophila, corresponds to aa 10 ... 234, 41% identity)

Expression and Regulation

Operons

• genes: [gene|BA60CF44ECA4937FA5C1720B10E7FDABA9AB4664|ybfO]-[gene|1EE8BF589ECDFD5194C86E02A627CCF81899B904|ybfP]-[gene|E06787B6AAD11070751938822F75359CE6EF299C|ybfQ]
  description: [Pubmed|12207695]

  sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|11866510,12207695], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|18840696,20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed during logarithmic growth ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]) [Pubmed|18840696]

Biological materials

Mutant

• MGNA-B936 (ybfQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1935 NBRP B. subtilis, Japan]
• BKE02330 (Δ[gene|E06787B6AAD11070751938822F75359CE6EF299C|ybfQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTAAACACCCTGTT, downstream forward: _UP4_TAAAAAAAGAACACCTCGTA
• BKK02330 (Δ[gene|E06787B6AAD11070751938822F75359CE6EF299C|ybfQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTAAACACCCTGTT, downstream forward: _UP4_TAAAAAAAGAACACCTCGTA

References

• 12207695